RGD peptide-modified chitosan as a gene carrier of implant surface
- VernacularTitle:RGD肽修饰壳聚糖作为种植体表面基因载体的研究
- Author:
Di ZHANG
1
;
Changhong LIU
;
Jincai ZHANG
;
Dehong CAI
;
Xiaoyu YANG
;
Shiyi LI
;
Huilan ZHONG
Author Information
- Keywords: RGD peptide; titanium; chitosan; implant
- From: West China Journal of Stomatology 2014;(4):336-340
- CountryChina
- Language:Chinese
- Abstract: Objective This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency. Methods An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy. The grafting effect of this complex on the titanium surface was detected by EB staining. Results CS and RGD peptides were coupled by an amide bond. The RGD-CS/pDNA complex was completely composited at N/P≥2. Atomic force microscopy results showed that the morphology of this complex was mainly spherical. EB staining experiments showed that this complex was successfully grafted on the titanium plate. Conclusion RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
