Effects of recombinant human erythropoietin on brain-derived neurotrophic factor expression in different brain regions of aging rats
10.3969/j.issn.1673-4254.2017.04.23
- VernacularTitle:重组人促红细胞生成素对衰老大鼠脑组织不同部位BDNF表达的影响
- Author:
Huqing WANG
1
;
Zhen GAO
;
Mengyi CHEN
;
Haiqin WU
;
Guilian ZHANG
;
Shuqin ZHAN
;
Ning BU
;
Jingjie LIU
;
Yuefen ZHAI
Author Information
1. 西安交通大学医学院第二附属医院神经内科
- Keywords:
brain-derived neurotrophic factor;
recombinant human erythropoietin;
aging;
nervous system
- From:
Journal of Southern Medical University
2017;37(4):551-554,562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of recombinant human erythropoietin (rhEPO) on expression of brain-derived neurotrophic factor (BDNF) in different brain regions of aging rats. Methods Forty male SD rats were randomized equally into negative control group, D-galactose group, EPO treatment group, and positive control group. Rat models of subacute aging were established by continuous subcutaneous injection of 5%D-galactose. Immunohistochemical staining was used to analyze the variation of BDNF expressions in different brain regions of the aging rats with different treatments. Results Significant brain region-specific differences in BDNF expression were found among the rats in different groups. Compared with those in the negative control group, the numbers of BDNF-positive cells in the hippocampal CA1 region, CA3 region, dentate gyrus (DG) and frontal cortex were all decreased obviously in D-galactose group (P<0.05) but increased in both EPO group and the positive control group (P<0.05) without significant differences between the latter two groups. In the rats in the same group, the number of BDNF-positive cells varied markedly in different brain regions (P<0.05), and the expression level of BDNF was the highest in the frontal cortex followed by the hippocampal CA3 region and the dentate gyrus, and was the lowest in the hippocampal CA1 region. Conclusion Treatment with rhEPO enhances the expression of BDNF in rat neural cells, suggesting that rhEPO may protect the nervous system from aging by regulating the BDNF pathway.
