Effect of Er：YAG laser on the dynamic changes in subgingival flora in type 2 diabetic patients with periodontitis
10.7518/hxkq.2014.02.015
- VernacularTitle:Er：YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究
- Author:
Bin GUO
1
;
Qianqian LIANG
;
Yan L
;
Min FU
;
Yuanyuan CHEN
;
Yifang YUAN
;
Yingchao LI
Author Information
1. 中国人民解放军总医院口腔医学研究所
- Keywords:
Er：YAG laser;
type 2 diabetic;
chronic periodontitis;
subgingival plaque;
denaturing gradient gel elec-trophoresis
- From:
West China Journal of Stomatology
2014;(2):171-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in subgingival microflora before and after Er: YAG laser treatment on dia-betic patients with periodontitis, and to compare with the subgingival microflora of chronic periodontitis. Methods Subgingival plaque of 13 pairs of teeth (26 sites) was selected from type 2 diabetic patients at pretreatment, one month post-treatment, and three months post-treatment. Subgingival plaque was also obtained from 11 cases of moderate to severe chronic periodon-titis with similar severity of periodontitis. The DNA of the subgingival plaque samples was extracted. Whole bacterial 16S rDNA gene fragments separated by denaturing gradient gel electrophoresis. Specific DNA bands were then chosen for retrieval and sequencing. Results The gene sequencing results of the special DNA bands of subgingival plaque samples show that the pathogenic bacteria of both diabetic periodontitis and simple chronic periodontitis were Prevotella intermedia and Tannerella forsythia, respectively. The composition of the subgingival microflora before and after laser treatment changed. Some DNA bands, including that of Tannerella forsythia, disappeared or weakened one month after treatment. A new strip appeared, which belonged to Actinomyces sp. Conclusion The profiles of the subgingival microflora changed after treatment, and one month was indicated as an important stage. Er: YAG laser may have an important function in delaying microflora recolonization.
