Polymorphism analysis of 20 autosomal short-tandem repeat loci in southern Chinese Han population
10.3969/j.issn.1673-4254.2017.02.01
- VernacularTitle:中国南方汉族人群20个常染色体STR基因座的多态性分析
- Author:
Ling CHEN
1
;
Huijie LU
;
Weian DU
;
Pingming QIU
;
Chao LIU
Author Information
1. 南方医科大学法医学院
- Keywords:
short-tandem repeat;
population genetics;
Han population
- From:
Journal of Southern Medical University
2017;37(2):141-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of PowerPlex（R）21 System (Promega) and study the genetic polymorphism of its 20 short-tandem repeat (STR) loci in southern Chinese Han population.Methods We conducted genotyping experiments using PowerPlex(R)21 System on 20 autosomal STR loci (D3S1358,D1S1656,D6S1043,D13S317,Penta E,D16S539,D18S51,D2S1338,CSF1PO,Penta D,TH01,vWA,D21S11,D7S820,D5S818,TPOX,D8S1179,D12S391,D19S433 and FGA) in 2367 unre1ated Chinese Han individuals living in South China.The allele frequencies and parameters commonly used in forensic science were statistically analyzed in these individuals and compared with the reported data of other populations.Results The PowerPlex（R） 21 System had a power of discrimination (PD) ranging from 0.7839 to 0.9852 and a power of exclusion (PE) ranging from 0.2974 to 0.8099 for the 20 loci.No significant deviation from Hardy-Weinberg expectations was found for all the loci except for D5S818.This southern Chinese Han population had significant differences in the allele frequencies from 8 ethnic groups reported in China,and showed significant differences at 8 to 20 STR foci from 5 foreign populations.The allele frequency at the locus D1S1656 in this southern Chinese Han population differed significantly from those in the 5 foreign populations and from 3 reported Han populations in Beijing,Zhejiang Province and Fujian Province of China.The neighbor-joining phylogenetictree showed clustering of all the Asian populations in one branch,while the northern Italian and Argentina populations clustered in a separate branch.This southern Chinese Han population had the nearest affinity with the Yi ethnic population in Yunnan Province of China.Conclusion The 20 STR loci are highly polymorphic in this southern Chinese Han population,suggesting the value of this set of STR loci in forensic personal identification,paternity testing and anthropological study.
