Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles
10.7518/hxkq.2014.02.006
- VernacularTitle:种植体周围炎对颌骨成骨细胞生物学功能的影响
- Author:
Hao WU
1
;
Hong-Chen LIU
;
Lingling E
;
Na LIU
;
Bo ZHANG
;
Yuxuan GAO
;
Lin FENG
Author Information
1. 中国人民解放军总医院口腔医学中心
- Keywords:
peri-implantitis;
osteoblast;
biological function
- From:
West China Journal of Stomatology
2014;(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts. Methods Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen Ⅰ (Col Ⅰ) mRNA levels were examined by real-time quantitative poly-merase chain reaction. OCN protein levels were determined by Western blot. Results After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue (P<0.05). After 7 d of culture, OCN, Runx2, and Col Ⅰ mRNA expression decreased (P<0.05). The OCN protein levels also decreased (P<0.05). Conclusion Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
