Effects of gossypol acetic acid on the proliferation and methylation level of the human MutL homologue 1 gene in human tongue carcinoma cell line Tca8113
10.7518/hxkq.2014.01.021
- VernacularTitle:醋酸棉酚对人舌鳞癌Tca8113细胞生长及人类错配修复基因1甲基化水平的影响
- Author:
Shuai FU
1
;
Yong WU
;
Qingfang PENG
;
Wenfei CHEN
Author Information
1. 昆明医科大学附属口腔医院口腔颌面外科
- Keywords:
Tca8113 cell;
gossypol;
tumor suppressor gene;
methylation
- From:
West China Journal of Stomatology
2014;(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective This paper aims to study the effects of gossypol acetic acid (GAA) on proliferation and methylation level of human MutL homologue 1 (hMLH1) gene in human tongue cancer cell line Tca8113. Methods The MTT assay was used to determine the effects of the acid on the proliferation inhibition in Tca8113 cells treated with different GAA concen-trations. Nested methylation-specific polymerase chain reaction (nMSP) was used to detect the change in the methylation level of hMLH1 after 48 and 72 h with 30 and 15μmol·L-1 GAA treatment. Results MTT assay results showed the growth and proliferation inhibition of Tca8113 cells in the experimental GAA group after 24 h to 72 h of GAA treatment. The nMSP results indicated that the average optical density of hMLH1 in the Tca8113 cells significantly changed after the GAA treat-ment (30μmol·L-1 GAA for 48 h and 15μmol·L-1 for 72 h) (P<0.05) compared with that of the control group. Conclusion GAA does not only inhibit Tca8113 proliferation but also has a demethylation effect on the hMLH1 gene. These phenomena may be part of an underlying tumor-suppression mechanism of GAA.
