MicroRNA- 133a antagonizes phenylephrine- induced hypertrophy of neonatal rat cardiomyocytes in vitro
- VernacularTitle:microRNA-133a拮抗苯肾上腺素诱导的小鼠心肌肥大
- Author:
Qi LI
1
;
Xiangsheng YANG
;
Xiaohua ZHOU
;
Lu XIAO
;
Xi LIN
;
Fengbo ZHANG
;
Lingli LI
;
Yanhong YU
;
Yanlin MA
Author Information
1. 海南医学院附属医院海南省人类生殖与遗传重点实验室
- Keywords:
microRNA-133a;
myocardial hypertrophy;
phenylephrine
- From:
Journal of Southern Medical University
2015;(9):1283-1286
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of miR-133a in reversing neonatal rat cardiomyocyte hypertrophy induced by phenylephrine. Methods A miR-133a precursor cDNA was used to construct an adenovirus vector, which was transfected into 293 cells to harvest miR-133a-containing virus. Neonatal rat cardiac myocytes treated by phenylephrine were exposed to miR-133a adenovirus, and the changes in cell area was measured; the expression levels of miR-133a and Acta1, Actc1, Actb, Myh6, Myh7, and BNP mRNAs were detected by quantitative RT-PCR. Results Phenylephrine treatment increased the area of cardiomyocytes by more than 3 folds and significantly enhanced the expression levels of Acta1, Actc1, Actb, Myh6, Myh7 and BNP mRNAs. All these changes were obviously reverse by miR-133a treatment. Conclusion miR-133a is an important regulator of phenylephrine-induced cardiomyocyte hypertrophy and negatively regulates this process
