Study on the function of keratinocyte growth factor on apoptosis of oral mucosal epithelial cells
10.7518/hxkq.2013.06.005
- VernacularTitle:角质细胞生长因子对口腔黏膜上皮细胞凋亡的作用研究
- Author:
Meirong WEI
1
;
Guo-Ju LI
;
Da ZHANG
;
Shiguo YAN
;
Xiangmin QI
Author Information
1. 山东大学口腔医院综合科
- Keywords:
keratinocyte growth factor;
apoptosis;
oral mucosal epithelial cells
- From:
West China Journal of Stomatology
2013;(6):565-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of keratinocyte growth factor (KGF) on apoptosis of oral mucosal epithelial cells and to provide a basis for further investigation of the role of KGF in the occurrence and development of oral mucosal diseases. Methods Different concentrations of KGF (control group, 0 ng·mL-1; experiment 1 group, 5 ng·mL-1; experimental 2 group, 25 ng·mL-1; experiment 3 group, 50 ng·mL-1) were added in oral mucosa epithelial cells cultured in vitro. After training for 12, 24, and 48 h, cell morphology was observed under an inverted microscope. Apoptosis was detected by using a flow cytometry instrument, and mRNA expression of apoptosis-related genes Bcl-2 and Bax was detected by using Real-Time fluorescent quantitative detection. Results Cell adherence of the experimental group was more obvious than that of the control group, and the cell nucleolus of the experiment 3 group was obviously cultured at 48 h. After culturing for 48 h, the apoptosis rate and Bcl-2 and Bax mRNA expression among the four groups were statistically significant. The increase of KGF concentration, apoptosis rate, and Bax mRNA expression gradually reduced, whereas Bcl-2 mRNA expression increased (P<0.05). Conclusion KGF may inhibit epithelial cell apoptosis through upregulation of Bcl-2 mRNA and downregulation of Bax mRNA.
