Cytotoxicity of gamma-ray in rat immature hippocampal neurons.
10.4142/jvs.2011.12.3.203
- Author:
Miyoung YANG
1
;
Myoung Sub SONG
;
Sung Ho KIM
;
Jong Choon KIM
;
Joong Sun KIM
;
Taekyun SHIN
;
Changjong MOON
Author Information
1. Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. moonc@chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
caspase;
cytotoxicity;
gamma-ray;
hippocampal neurons;
oxidative stress
- MeSH:
Amifostine/pharmacology;
Animals;
Antioxidants/pharmacology;
Caspase 3/metabolism/radiation effects;
Catechin/analogs & derivatives/pharmacology;
Cell Survival/radiation effects;
Cells, Cultured/cytology/enzymology/*radiation effects;
Dose-Response Relationship, Radiation;
Female;
*Gamma Rays;
Hippocampus/cytology/enzymology/*radiation effects;
L-Lactate Dehydrogenase/radiation effects;
Neurons/cytology/enzymology/*radiation effects;
Poly(ADP-ribose) Polymerases/drug effects;
Pregnancy;
Rats;
Rats, Sprague-Dawley
- From:Journal of Veterinary Science
2011;12(3):203-207
- CountryRepublic of Korea
- Language:English
-
Abstract:
This in vitro study evaluated the detrimental effect of acute gamma (gamma)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy gamma-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after gamma-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked gamma-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of gamma-rays in immature hippocampal cultured neurons may be caused by oxidative stress.
