Impact of Interstitial Irradiation with 32 P Glass Microspheres on Cellular Apoptosis of Mice with the Solid Tumor S180
- VernacularTitle:32磷玻璃微球组织内照射对肿瘤细胞 凋亡影响的实验研究
- Author:
Yongyue WANG
1
;
Dazhang WANG
;
Guangyong ZHENG
Author Information
1. West China University of Medical Sciences
- From:
West China Journal of Stomatology
2001;19(2):116-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of this study is to observe apoptotic changes of tumor cells in mice with the solid tumor S180 after interstitial irradiation with 32 P glass microspheres.Methods: Twenty mice with solid tumor S180 were divided into four groups. The control group was given normal saline. The experimental groups were given different doses of 32 P glass microspheres (50, 150, 450uci per mouse) using interstitial implant. The terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method was used to determine the apoptotic cells. Results: The mean values of the apoptotic indexes of these groups were respectively 0.39, 0.41, 0.59 and 0.95. The apoptotic indexes of the second and the third experimental groups were significantly higher than that of the control group (P<0.05).Conclusion: The interstitial irradiation with 32 P glass microspheres can enhance the activity of apoptotic cells of solid tumor S180, and the effect is dose_dependent.
