Determination of staphylococcal exotoxins, SCCmec types, and genetic relatedness of Staphylococcus intermedius group isolates from veterinary staff, companion animals, and hospital environments in Korea.
10.4142/jvs.2011.12.3.221
- Author:
Jung Ho YOUN
1
;
Hye Cheong KOO
;
Kuk Ju AHN
;
Suk Kyung LIM
;
Yong Ho PARK
Author Information
1. Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. koohj@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
methicillin-resistant Staphylococcus intermedius group;
Staphylococcus intermedius group;
Staphylococcus pseudintermedius;
toxin
- MeSH:
Animals;
Bacterial Toxins/genetics/metabolism;
Cat Diseases/epidemiology/*microbiology;
Cats;
Chromosomes, Bacterial/genetics/metabolism;
Dog Diseases/epidemiology/*microbiology;
Dogs;
Electrophoresis, Gel, Pulsed-Field/veterinary;
Enterotoxins/genetics/metabolism;
Exfoliatins/genetics/metabolism;
Exotoxins/*genetics/metabolism;
Hospitals, Animal;
Humans;
Medical Staff, Hospital;
Molecular Sequence Data;
Pets/microbiology;
Polymerase Chain Reaction/veterinary;
Republic of Korea/epidemiology;
Staphylococcal Infections/epidemiology/microbiology/*veterinary;
Staphylococcus/genetics/isolation & purification;
Staphylococcus intermedius/*genetics/*isolation & purification
- From:Journal of Veterinary Science
2011;12(3):221-226
- CountryRepublic of Korea
- Language:English
-
Abstract:
The Staphylococcus (S.) intermedius group (SIG) has been a main research subject in recent years. S. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. To prevent SIG-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (SCCmec) types among SIG isolates may be helpful. In this study, it was found that a single isolate (one out of 178 SIG isolates examined) harbored the canine enterotoxin SEC gene. However, the S. intermedius exfoliative toxin gene was found in 166 SIG isolates although the S. aureus-derived exfoliative toxin genes, such as eta, etb and etd, were not detected. SCCmec typing resulted in classifying one isolate as SCCmec type IV, 41 isolates as type V (including three S. intermedius isolates), and 10 isolates as non-classifiable. Genetic relatedness of all S. pseudintermedius isolates recovered from veterinary staff, companion animals, and hospital environments was determined by pulsed-field gel electrophoresis. Strains having the same band patterns were detected in S. pseudintermedius isolates collected at 13 and 18 months, suggesting possible colonization and/or expansion of a specific S. pseudintermedius strain in a veterinary hospital.
