MicroRNA-34a regulates cell cycle by targeting CD44 in human bladder carcinoma cells
10.3969/j.issn.1673-4254.2015.07.02
- VernacularTitle:微小RNA-34a通过靶向CD44调节膀胱癌细胞J82周期
- Author:
Gan YU
1
;
Kai XU
;
Shian XU
;
Xiaolan ZHANG
;
Qianhua HUANG
;
Bin LANG
Author Information
1. 华中科技大学同济医学院附属同济医院泌尿外科
- Keywords:
miR-34a;
CD44;
cell cycle;
bladder cancer
- From:
Journal of Southern Medical University
2015;(7):935-940
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of microRNA-34a (miR-34a) in regulating the cell cycles of bladder cancer cell line J82 and explore the underlying mechanism. Methods J82 cells were transfected with a miR-34a mimic or an inhibitor to induce miR-34a overexpression or silencing. The RNA level of miR-34a in the transfected cells was detected by real-time PCR, and CD44 expressions at the mRNA and protein levels were detected by real-time PCR and Western blotting. Luciferase reporter assay was used to detect the activation of 3'UTR of CD44, and flow cytometry was performed to analyze the cell cycle changes. Results The expression level of miR-34a was significantly increased and CD44 expression significantly lowered in cells transfected with miR-34a mimic; miR-34a inhibitor transfection caused reverse effects on miR-34a and CD44 expressions. MiR-34a mimics downregulated while miR-34a inhibitor enhanced the activation of 3'UTR of CD44 with corresponding changes in the expressions of some cell cycle-related proteins. MiR-34a mimics and miR-34a inhibitor induced opposite changes in J82 cell cycle, which were partly reversed by CD44. Conclusion MiRNA-34a regulates cell cycles by targeting CD44 in human bladder carcinoma cell line J82.
