Observation of radiobiological characteristics in a HepG2 cell line with mitochondrial DNA deletion
- VernacularTitle:线粒体DNA缺失HepG2细胞系的建立、鉴定及放射生物学特性
- Author:
Hengwen SUN
1
;
Yi PAN
;
Zijun ZENG
;
Liangyi FANG
;
Hongdan ZHANG
;
Songxi XIE
;
Weixiong LI
;
Jiabin XU
Author Information
1. 广东省人民医院//广东省医学科学院肿瘤中心放疗科
- Keywords:
radiosensitivity;
HepG2;
Rho0 cells;
apoptosis
- From:
Journal of Southern Medical University
2015;(6):783-788
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiobiological characteristics of a HepG2 cell line with mitochondrial DNA (mtDNA) deletion. Methods HepG2 cells were cultured in a medium containing ethidium bromide, acetylformic acid and uracil. The HepG2 cell line with mtDNA deletion (ρ0HepG2 cells) were acquired after 30 subcultures by limited dilution cloning. The cell survival was then observed in the absence of acetylformic acid and uracil, and the total mtDNA deletion in the cells was confirmed by PCR. The radiosensitivity of HepG2 andρ0HepG2 cells was evaluated by exposure to gradient doses of 6 MV X ray irradiation. The cell apoptosis was assessed following a 2 Gy X-ray exposure with Hochest33342 staining, and the invasiveness of ρ0HepG2 cells was measured by Transwell assay. Results HepG2 cells could survive 30 subcultures in the presence of ethidium bromide, and massive cell death occurred after removal of acetylformic acid and uracil from the medium. PCR confirmed total mtDNA deletion fromρ0HepG2 cells, whoseα/βvalue was significantly lower than that of HepG2 cells.ρ0Hep-G2 cells showed an obviously lowered cell apoptosis rate following X-ray exposure with enhanced cell invasiveness. Conclusion HepG2 cells can be induced by ethidium bromide intoρ0HepG2 cells with an increased radiation resistance, anti-apoptosis ability and cell invasiveness.
