Effects of different concentrations of putrescine on proliferation, migration and apoptosis of human skin fibroblasts
10.3969/j.issn.1673-4254.2015.05.27
- VernacularTitle:不同浓度腐胺对人皮肤成纤维细胞增殖、迁移和凋亡的影响
- Author:
Jianxia CHEN
1
;
Xinzhou RONG
;
Guicheng FAN
;
Songze LI
;
Qinghui LI
Author Information
1. 广州医科大学附属广州市第一人民医院烧伤外科
- Keywords:
putrescine;
proliferation;
migration;
apoptosis;
human skin fibroblasts
- From:
Journal of Southern Medical University
2015;(5):758-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different concentrations of putrescine on the proliferation, migration and apoptosis of human skin fibroblasts (HSF). Methods HSF cultured in the presence of 0.5, 1.0, 5.0, 10, 50, 100, 500, and 1000μg/ml putrescine for 24 h were examined for the changes in the cell proliferation, migration, and apoptosis using MTS assay, Transwell migration assay, and flow cytometry, respectively. Results Compared with the control cells, HSF cultured with 0.5, 1.0, 5.0, and 10μg/ml putrescine showed significantly increased cell proliferation (P<0.01), and the effect was the most obvious with 1 μg/ml putrescine, whereas 500 and 1000 μg/ml putrescine significantly reduced the cell proliferation (P<0.01); 50 and 100 μg/ml did not obviously affect the cell proliferation (P>0.05). Putrescine at 1 μg/ml most significantly enhanced the cell migration (P<0.01), while at higher doses (50, 100, 500, and 1000μg/ml) putrescine significantly suppressed the cell migration (P<0.05);0.5, 5.0, and 10μg/ml putrescine produced no obvious effects on the cell migration (P>0.05). HSF treated with 0.5, 1.0, 5.0, and 10 μg/ml putrescine obvious lowered the cell apoptosis rate compared with the control group (P<0.01), and the cell apoptosis rate was the lowest in cells treated with 1μg/ml putrescine;but at the concentrations of 100, 500, and 1000μg/ml, putrescine significantly increased the cell apoptosis rate (P<0.01), while 50μg/ml putrescine produced no obvious effect on cell apoptosis (P>0.05). Conclusion Low concentrations of putrescine can obviously enhance the proliferation ability and maintain normal migration ability of HSF in vitro, but at high concentrations, putrescine can obviously inhibit the cell migration and proliferation and induce cells apoptosis, suggesting the different roles of different concentrations of putrescine in wound healing.
