Effects of chloroquine in reversing multidrug resistance in HNE1/DDP cell line
10.3969/j.issn.1673-4254.2015.05.12
- VernacularTitle:氯喹逆转人鼻咽癌细胞HNE1/DDP的耐药作用
- Author:
Haoxuan ZHANG
1
;
Xiaojin SUN
;
Yiming SUN
;
Surong ZHAO
;
Chenchen JIANG
;
Hao LIU
Author Information
1. 蚌埠医学院 基础医学部
- Keywords:
nasopharyngeal carcinoma;
chloroquine;
multi-drug resistance;
MDR1;
P-glycoprotein
- From:
Journal of Southern Medical University
2015;(5):687-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of chloroquine in reversing multidrug resistance (MDR) of HNE1/DDP cell line and explore the mechanism. Methods MTT assay was used to detect the cell viability of HNE1 and HNE1/DDP after exposure to different concentrations of DDP (2, 4, 8, 16 and 32μmol/L) and different concentrations of chloroquine (5, 10, 20, 40, and 80μmol/L). q-PCR was used to assess the expression of MDR1 mRNA and Western blotting was employed to detect P-glycoprotein (P-gp) expression in HNE1 and HNE1/DDP cells exposed to 5 and 10μmol/L chloroquine. The cell apoptosis rate of HNE1 and HNE1/DDP cells exposed to 10 and 20μmol/L chloroquine was determined by PI assay. Results Chloroquine exposure caused dose-dependent suppression of the proliferation in both HNE1 and HNE1/DDP cells, and significantly reversed multidrug resistance in HNE1/DDP cells. The expressions of MDR1 mRNA and P-gp protein were significantly lowered in the cells treated with chloroquine. Conclusion Chloroquine can reverse multidrug resistance in HNE1/DDP cells possibly through down-regulation of MDR1 and inhibition of P-gp protein.
