Effects of hydrogen sulfide on myocardial fibrosis and MAPK1/3 and MMP-8 expression in diabetic rats
- VernacularTitle:硫化氢对糖尿病大鼠心肌纤维化及MAPK1/3和MMP-8表达的影响
- Author:
Fang LI
1
;
Ou ZENG
;
Jian LUO
;
Zhixiong WU
;
Ting XIAO
;
Jingjing ZHANG
;
Jun YANG
Author Information
1. 南华大学附属第一医院心血管内科
- Keywords:
hydrogen sulfide;
diabetes mellitus;
myocardial fibrosis;
collagen I;
MAPK1/3;
MMP-8
- From:
Journal of Southern Medical University
2015;(4):549-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hydrogen sulfide (H2S) on myocardial fibrosis and expressions of MAPK1/3 and MMP-8 in diabetic rats. Methods Forty adult male SD rats were randomized into 4 groups, namely the control group, diabetes mellitus group (STZ group), diabetes mellitus with H2S treatment group (STZ+H2S group), and normal rats with H2S treatment group (H2S group). Diabetes was induced by intraperitoneal injections of 40 mg/kg streptozotocin (STZ). The rats in the control group received daily intraperitoneal injections of saline, and those in STZ+H2S group and H2S group were given NaHS (100μmol/kg) injections. After 8 weeks, the pathologies of cardiac fibrosis were examined with HE staining, and the expressions of collagen I, MAPK1/3 and MMP-8 were analyzed with Western blotting. Results Compared with the control group, the diabetic rats showed increased collagen content and obvious interstitial fibrosis in the myocardial tissue with significantly increased expression levels of collagen I, MAPK1/3 and MMP-8 (P<0.05); all these changes were obviously reversed by treatment with H2S (P<0.05). Collagen I, MAPK1/3 and MMP-8 expression levels and the degree of myocardial fibrosis were comparable between H2S group and control group (P>0.05). Conclusion Hydrogen sulfide can attenuate cardiac fibrosis in diabetic rats, and the mechanism may involve the inhibition of MAPK1/3/MMP-8 signal pathway.
