Effect of resveratrol on constrictions of human intrapulmonary arteries ex vivo
- VernacularTitle:白藜芦醇对离体人肺内小动脉血管张力的影响
- Author:
Zhaojun WANG
1
;
Chunyu DENG
;
Sujuan KUANG
;
Lidan NONG
;
Guangyan ZHANG
;
Jue MA
;
Jianxiu CUI
Author Information
1. 南方医科大学
- Keywords:
resveratrol;
human intrapulmonary arteries;
relaxation;
endothelium
- From:
Journal of Southern Medical University
2015;(4):540-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of resveratrol on constrictions of isolated human intrapulmonary arteries and its mechanisms. Methods Intrapulmonary arteries (1-1.5 mm in diameter) were dissected and cut into rings (1.8-2.0 mm in length) under microscope, and were then mounted in a Multi Myograph system. The rings were stimulated with 100 nmol/L U46619, 30 nmol/L endothelin-1, or 60 mmol/L KCl to produce sustained contraction of the intrapulmonary arteries, after which resveratrol was applied cumulatively. Endothelium denudation, L-NAME and indomethecin were used to investigate the effect of resveratrol on constrictions of the isolated arteries, suing DMSO as the control. Results Resveratrol induced concentration-dependent relaxations in endothelium-intact rings that contracted in response to stimulations with U46619, ET-1 and KCl, with pD2 of 3.82 ± 0.20, 3.84 ± 0.57, and 3.68 ± 0.27, Emax of (99.58 ± 0.83)%, 100%, and (99.65 ± 0.98)%, respectively. Treatment of the arterial rings with the eNOS inhibitor L-NAME, but not with indomethecin or endothelium denudation, obviously affected the relaxant effects of resveratrol. Conclusion Resveratrol can concentration-dependently produce relaxant effect on human intrapulmonary arteries independent of the endothelium possibly by promoting synthesis and release of NO.
