Screening of highly efficient small interfering RNAs against bone morphogenetic protein-7
10.3969/j.issn.1673-4254.2015.03.18
- VernacularTitle:高效能沉默BMP-7基因siRNA序列的实验筛选
- Author:
Caixia LI
1
;
Peng BAI
;
Xian ZHAO
;
Yuan DONG
;
Xiaoming DAI
;
Yisong LI
;
Liu LIU
Author Information
1. 昆明医科大学
- Keywords:
bone morphogenetic protein-7;
screening;
small interfering RNA
- From:
Journal of Southern Medical University
2015;(3):403-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain highly efficient small interfering RNAs (siRNAs) against bone morphogenetic protein-7 (BMP-7) for future functional analysis of BMP-7 in bone homeostasis. Methods We designed 4 independent siRNA sequences against rat BMP-7 and transfected them in rat umbilical vein endothelial cells. After 72 h, we examined BMP-7 mRNA and protein level in the transfected cells using real-time PCR and Western blotting, respectively. Results All the 4 siRNAs effectively reduced BMP-7 expression in rat umbilical vein endothelial cells, and among them BMP-7-3 siRNA showed the highest efficiency. Conclusion We have obtained an efficient siRNA to knockdown BMP-7 expression in vitro for further investigation of the role of BMP-7 in bone and cartilage formation.
