Effect of anacardic acid, a Hsp90 inhibitor, on proliferation, invasion and migration of breast cancer MDA-MB-231 cells
10.3969/j.issn.1673-4254.2015.03.08
- VernacularTitle:Hsp90抑制剂槚如酸对乳腺癌MDA-MB-231细胞增殖、侵袭、迁移的影响
- Author:
Hongmei LI
1
;
Lijuan NIE
;
Qiang HUO
;
Surong ZHAO
;
Tao MA
;
Chengzhu WU
;
Hao LIU
Author Information
1. 蚌埠医学院药学系
- Keywords:
anacardic acid;
Hsp90;
triple negative breast cancer;
anti-tumor activity
- From:
Journal of Southern Medical University
2015;(3):355-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the Hsp90 inhibitor anacardic acid on cell proliferation, invasion and migration of breast cancer MDA-MB-231 cells. Methods The inhibitory effect of anacardic acid on Hsp90 was assessed with in vitro ATPase inhibition assay and ATP-sepharose binding assay. MTT assay was used to detect the growth inhibition induced by anacardic acid in MDA-MB-231 cells. Transwell assays were used to evaluate MDA-MB-231 cell invasion and migration. Western blotting was performed to assess the effect of anacardic acid in triggering the degradation of MMP-9, TIMP-1, Hsp90, and Hsp70. Results Anacardic acid exhibited a modest activity of ATPase inhibition with an IC50 value of 82.5 μmol/L. Anacardic acid significantly suppressed the proliferation of MDA-MB-231 cells in a dose-dependent manner (IC50 value of 29.3 μmol/L). Treatment with 12.5, 25, and 50μmol/L anacardic acid for 36 h caused inhibition of cell invasion by 23.6%, 56.6%, and 67.0%in MDA-MB-231 cells, respectively (P<0.05), and anacardic acid treatment for 24 h inhibited the cell migration by 30.0%, 45.5%, and 77.5%, respectively (P<0.05). Anacardic acid dose-dependently induced MMP-9 degradation, but did not obviously affect Hsp90 or Hsp70 expressions. Conclusion Anacardic acid can significantly inhibit the proliferation, invasion, and migration of MDA-MB-231 cells, the mechanism of which may involve the inhibition of Hsp90 ATPse activity and down-regulation of MMP-9 expression.
