Expression of transient receptor potential canonical 1 in ozone-induced inflammatory lung tissues in mice
10.3969/j.issn.1673-4254.2015.02.27
- VernacularTitle:瞬时感受器电位通道1在臭氧致小鼠肺组织炎症中的表达
- Author:
Zhaodi FU
1
;
Lifen ZHOU
;
Jianrong HUANG
;
Shuyi GUO
;
Jiechun ZHANG
;
Yongbiao FANG
;
Xiaoai LIU
;
Qingzi CHNE
;
Jianhua LI
Author Information
1. 广州医科大学生理学教研室
- Keywords:
transient receptor potential canonical 1;
ozone;
lung;
inflammation
- From:
Journal of Southern Medical University
2015;(2):284-287,291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of ozone-induced lung inflammation and explore its role in lung inflammation. Methods In a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern blotting and immunohistochemistry. Results Compared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice (P<0.01). Conclusion TRPC1 may play a role in ozone-induced lung inflammation in mice.
