Preparation and characterization of uricase in uricase-catalase liposomes prepared using bo-rate buffer
10.3969/j.issn.1673-4254.2015.02.23
- VernacularTitle:硼酸盐缓冲液制备尿酸酶复合脂质体及尿酸酶的特性
- Author:
Yunli ZHOU
1
;
Lin YANG
;
Zijun YAN
;
Xue DENG
;
Jingqing ZHANG
Author Information
1. 重庆医科大学药物高校工程研究中心
- Keywords:
uricase;
catalase;
liposomes;
michaelis-menten constand;
fluorescence spectrum
- From:
Journal of Southern Medical University
2015;(2):268-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer. Methods BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined. Results The optimal temperature of BUCLP and URI was 40 oC, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 μmol/L and 13.623 μmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm. Conclusion Uricase activity is enhanced after loading in uricase and catalase liposomes.
