Role of KA1 receptor in excitotoxic neurodegeneration in mouse hippocampus triggered by kainic acid-or tunicamycin-induced endoplasmic reticulum stress
10.3969/j.issn.1673-4254.2015.02.07
- VernacularTitle:KA1亚受体在内质网应激致海马神经元死亡中的作用
- Author:
Lei YUAN
1
;
Jiqin GONG
;
Haixia ZHANG
;
Shilei QIAN
;
Bin XU
;
Jie ZENG
;
Juan ZHAO
;
Huaxu YU
Author Information
1. 长沙医学院第一临床学院2010级
- Keywords:
KA1 subunit,kainate receptor;
kainic acid;
tunicamycin;
endoplasmic reticulum stress;
diabetic encephalopathy;
hippocampus;
brain in stereotaxic;
bederson score;
immunohistochemistry
- From:
Journal of Southern Medical University
2015;(2):191-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of up-regulation of KA1 subunit of the kainate receptor on endoplasmic reticulum stress (ERS)-induced excitotoxic neurodegeneration in mouse hippocampus. Methods Seventy adult male KM mice were subjected to microinjections into the hippocampus of kainic acid (KA) or 500, 1000, or 2000μg/ml tunicamycin (TM). At 1, 2, 3, 4, 5, 8, and 12 h after the injections, the mice were assessed for Bederson scores and sacrificed for FJB staining and immunofluorescence observation of the brain slices. Results At 3, 4, 5, and 8 h after KA injection and at 4 and 5 h after of 2000μg/ml TM injection, the mice showed severe central nervous system dysfunction, and FJB staining revealed increased cell death in the hippocampus, where up-regulated expressions of KA1 receptor and ERS marker P-eIF2α were found by immunofluorescence staining (P<0.05). Conclusion Microinjection of KA or TM into the hippocampus causes neuronal death and ERS with up-regulated expression of KA1. In this process of neuronal apoptosis, the membrane receptor KA1 receives the apoptosis signal and transfers it to the inside of the cells to cause cell endoplasmic reticulum dysfunction and ERS response, which ultimately leads to neuronal death.
