Effects of electro-acupuncture on expression of triggering receptor expressed on myeloid cells 1 in ankle joint synovial tissue of acute gouty arthritis rats
10.3969/j.issn.1673-4254.2015.01.27
- VernacularTitle:电针对急性痛风性关节炎大鼠踝关节髓样细胞表达激发受体表达的影响
- Author:
Chaonan ZHANG
1
;
Xuekuan HUANG
;
Yan LUO
;
Juan JIANG
;
Lei WAN
;
Ling WANG
Author Information
1. 重庆医科大学中医药学院
- Keywords:
acute gouty arthritis;
electro-acupuncture;
triggering receptor expressed on myeloid cells1
- From:
Journal of Southern Medical University
2015;(1):133-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of electro-acupuncture (EA) on the expression of triggering receptor expressed on myeloid cell(TREM)1 in ankle joint synovial tissue of acute gouty arthritis (AGA) rats. Methods Forty male SD rats were randomly divided into 4 groups:normal, AGA, medication and EA group, 10 rats in each group. AGA model was established by induced monosodium urate (MSU) method, except the normal group. Tow days before AGA model was established, normal and AGA groups were lavaged with normal saline (20 ml/kg), medication group was lavaged with colchicine solution (20 ml/kg), EA (1.5-2Hz, D.-D.wave, 9v, 1-3 mA) was applied to“Sanyinjiao”(SP6),“jiexi”(ST41) and“Kunlun”(BL60) for 20 min, once daily, continuously for 9 days. Then observed the changes in dysfunction, and the content of TNF-αand IL-1βdetected by ELISA ,the expression of TREM-1 detected by immunohistochemistry and western blot. Results Compared to the normal group, the AGA group of the dysfunction index increased significantly (P<0.01), the content of TNF-αand IL-1βincreased significantly (P<0.05), the expression of TREM-1 in synovial tissue increased significantly (P<0.05); the medication and EA groups compared to the AGA group, the dysfunction index decreased significantly (P<0.01), the content of TNF-αand IL-1βdecreased significantly (P<0.05), the expression of TREM-1 in synovial tissue decreased significantly (P<0.05); there were not statistically significant between the medication and EA group (P>0.05). Conclusion EA treating AGA may be through down-regulating the expression of TREM-1 in synovial tissue.
