Effect of clopidogrel on plasma protein binding rate of ginsenosides：a liquid chromatography-mass spectrometry-based study
10.3969/j.issn.1673-4254.2015.01.21
- VernacularTitle:基于液相色谱质谱联用氯吡格雷-人参皂苷血浆蛋白结合率协同效应
- Author:
Shitang MA
1
;
Guoliang DAI
;
Wenzhu ZHAO
;
Bingting SUN
;
Wenzheng JU
;
Hengshan TAN
Author Information
1. 安徽科技学院食品药品学院
- Keywords:
clopidogrel;
ginsenosides;
plasma protein binding rate
- From:
Journal of Southern Medical University
2015;(1):109-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of clopidogrel on the binding rate of ginsenosides with rat serum proteins (RSA). Methods Equilibrium dialysis and liquid chromatography-mass spectrometry were employed to quantify the concentration of ginsenoside Rg1 and Rb1. The protein-binding rates of Rg1 and Rb1 in the presence or absence of clopidogrel (1.0 mg/L) were determined. A molecular simulation model (consisting of homology modeling and molecular docking interaction) was used to reveal the target protein-compound interactions. Results The binding rates of ginsenosides Rg1 (0.4, 1.0, and 2.0 mg/L) with RSA were (30.16±2.82)%, (33.42±4.21)%, and (34.61±3.42)%, and those of and Rb1 were (50.13±2.34)%, (51.23±3.23)%, and (53.11± 3.26)%, respectively. In the presence of clopidogrel, the binding rates of Rg1 decreased to (22.13 ± 2.72)%, (21.42 ± 3.22)%, and (25.45 ± 3.52)%, and those of Rb1 to (40.13 ± 3.24)%, (41.25 ± 4.15)%, and (43.11 ± 3.31)%, receptively. The molecular docking suggested that these compounds competed to bind with RSA. Conclusion Clopidogrel can competitively bind to RSA with ginsenosides to lower the plasma protein binding rates of ginsenosides.
