Mitochondrial estrogen receptorβinhibits non-small cell lung cancer cell apoptosis via inter-action with Bad
10.3969/j.issn.1673-4254.2015.01.19
- VernacularTitle:线粒体雌激素受体β通过与Bad相互作用抑制非小细胞肺癌细胞的凋亡
- Author:
Qiang XIE
1
;
Zuoping HUANG
;
Ying LIU
;
Xiao LIU
;
Lei HUANG
Author Information
1. 武警广东省总队医院
- Keywords:
estrogen receptorβ;
mitochondria;
non-small cell lung cancer;
Bad;
Bax;
apoptosis
- From:
Journal of Southern Medical University
2015;(1):98-102,108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanisms by which mitochondrial estrogen receptorβ(ERβ) suppresses non-small cell lung cancer cell apoptosis induced by apoptotic stimulations. Methods The mitochondrial localization of ERβin non-small cell lung cancer cell lines A549 and 201T was determined using immunofluorescence and Western blotting. The changes of apoptosis of the cells with mitochondrial ERβ overexpression or knockdown in response to cisplatin and STS treatments were assessed, and mitochondrial ERβ interaction with the pro- apoptotic protein Bad was detected using co-immunoprecipitation and Western blotting. Results ERβ was localized in the mitochondria in A549 and 201T cells. ERβoverexpression significantly reduced while ERβknockdown increased Bax activation and cell apoptosis induced by cisplatin and STS. Mitochondrial ERβinteraction with pro-apoptotic protein Bad may suppress Bax activation and its translocation to the mitochondria. Conclusion Mitochondrial ERβ can suppress apoptosis of non-small cell lung cancer cells induced by cisplatin or STS through interaction with Bad, suggesting the value of mitochondrial ERβ as a new therapeutic target for treatment of non-small cell lung cancer.
