Cisplatin inhibits proliferation of cervical carcinoma cell line by up- regulating Stat1 expression
10.3969/j.issn.1673-4254.2015.01.17
- VernacularTitle:顺铂上调STAT1蛋白抑制宫颈癌细胞增殖
- Author:
Ruilin LEI
1
;
Songshu XIAO
;
Min XUE
Author Information
1. 中南大学湘雅三医院妇科
- Keywords:
Stat1;
c-Myc;
cervical carcinoma;
HeLa cells;
cisplatin;
chemotherapy
- From:
Journal of Southern Medical University
2015;(1):88-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Stat1 gene in the proliferation and chemotherapeutic sensitivity of cervical cancer HeLa cells. Methods The protein expression of Stat1 in the Hela cells exposed to gradient concentrations of cisplatin (DDP) was detected by Western blotting with or without small interfering RNA (siRNA)-mediated Stat1 gene silencing. The effect of Sata1 silencing on the sensitivity to DDP and cell proliferation of the cells was tested using MTT assay and BrdU assay, and the expression of c-Myc was detected by Western blotting in the cells treated with siRNA and DDP. Results The expression of Stat1 in Hela cells exposed to DDP increased with the DDP concentrations, reaching 1.5 folds of the baseline at a DDP concentration of 5 mg/L and 2 folds at 10 mg/L. Stat1-siRNA effectively reduced Stat1 expression in Hela cells, promoted the cell proliferation, and enhanced the expression of c-Myc;DDP inhibited the cell growth and down-regulated c-Myc expression. Stat1-siRNA rescued DDP-induced inhibition of cell growth and c-Myc down-regulation. Conclusion The expression of Stat1 is associated with DDP sensitivity in cervical cancer cells, and Stat1 silencing can increase the sensitivity to DDP and c-Myc expression of the cells.
