Placental Foxp3 expression in patients with preeclampsia and correlation of Foxp3 gene lo-cus 924 (rs2232365) polymorphism with preeclampsia
10.3969/j.issn.1673-4254.2015.01.15
- VernacularTitle:子痫前期与Foxp3表达量及其基因位点924（rs2232365）多态性的相关性
- Author:
Ximing CHEN
1
;
Wei XU
;
Yongquan CHEN
;
Zhiqiong LIAO
;
Ting GAN
;
Aihua WU
;
Degui LIAO
;
Guohong XIAO
;
Shengqiang CHEN
Author Information
1. 广州医科大学附属第三医院
- Keywords:
preeclampsia;
Foxp3-924;
polymorphisms
- From:
Journal of Southern Medical University
2015;(1):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect changes of Foxp3 expression in the decidua in patients with preeclampsia and investigate the correlation of Foxp3-924 (rs2232365) polymorphisms with preeclampsia. Methods From October 2011 to December 2012, 252 normal pregnant women and 156 preeclampsia patients of Han nationality from the same geographic region were tested for Foxp3-924 genotypes by polymerase chain reaction with sequence-specific primer (PCR-SSP). Sixty-eight of the patients with preeclampsia (33 with mild and 35 with severe preeclampsia) and 30 of the normal pregnant women were also examined for Foxp3 expression in the decidua using immunohistochemical method. Results Foxp3 positive expression rates in the decidua was 51.52%in mild preeclampsia and 28.57%in severe preeclampsia cases, significantly lower than that in the control group (86.67%, P<0.05). In preeclampsia patients, the frequencies of Foxp3-924G/G, G/A, and A/A genotypes were 0.1346, 0.4615 and 0.4038, respectively, and the frequencies of Foxp3-924A and Foxp3-924 G were 0.6346 and 0.3654, respectively. The genotype frequencies of Foxp3-924G/G, G/A and A/A in the control group were 0.1508, 0.4087 and 0.4405, respectively, and the frequencies of Foxp3-924 A and Foxp3-924 G were 0.6448 and 0.3552, respectively. No significant differences were found in the gene frequencies of Foxp3-924G/A between preeclampsia patients and the control group (P>0.05). Conclusion The expression level of Foxp3 in the placental tissue of preeclampsia patients is significantly lower than that in normal pregnant women, suggesting that lowered Foxp3 expression decreases the immunosuppressive function and causes imbalance of immune tolerance between maternal-fetal to induce preeclampsia. Foxp3-924 polymorphisms is not significantly correlated with the occurrence of preeclampsia.
