Sodium valproate enhances doxorubicin cytotoxicity in breast cancer cells in vitro
10.3969/j.issn.1673-4254.2015.01.12
- VernacularTitle:丙戊酸钠增强阿霉素的体外抗乳腺癌作用
- Author:
Xuhui TONG
1
;
Chao ZHENG
;
Guojun JIANG
;
Shuying DONG
Author Information
1. 蚌埠医学院药学系药理学教研室
- Keywords:
breast cancer;
sodium valproate;
doxorubicin;
apoptosis
- From:
Journal of Southern Medical University
2015;(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sodium valproate, a histone deacetylase inhibitor, on the cytotoxicity of doxorubicin in breast cancer cells. Methods Western blotting was used to assess Cx43 protein expression in breast cancer Hs578T cells exposed to doxorubicin and sodium valproate. MTT assay was used to determine the cytotoxicity of doxorubicin;annexin V/PI double staining and Hochest 33258 fluorescence staining were employed to detect doxorubicin-induced early and late apoptosis, respectively. Results Western blotting showed that sodium valproate significantly increased Cx43 protein expression in Hs578T cells (P<0.01). The cells exposed to both sodium valproate and doxorubicin showed significantly lowered cell viability compared with the cells exposed to doxorubicin alone (P<0.01). Exposure to both sodium valproate and doxorubicin resulted in significantly increased early and late cell apoptosis rate compared with doxorubicin treatment alone (P<0.01). Conclusion sodium valproate can significantly enhance the cytotoxicity of doxorubicin and increase doxorubicin-induced apoptosis in breast cancer cells in vitro possibly by enhancing the gap junction function.
