Inhibitory effect of chloroquine on airway hyperresponsiveness in asthmatic mice
10.3969/j.issn.1673-4254.2015.01.03
- VernacularTitle:氯喹对哮喘小鼠气道高反应性的抑制作用
- Author:
Xiaochun SUN
1
;
Xiaoyan HU
;
Lijia WANG
;
Enmei LIU
;
Zhou FU
Author Information
1. 重庆医科大学附属儿童医院呼吸病学实验室
- Keywords:
asthma;
chloroquine;
airway hyperresponsiveness;
interleukin-6;
PGF2α
- From:
Journal of Southern Medical University
2015;(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of chloroquine on airway hyperresponsiveness in asthmatic mice and explore the possible mechanism. Methods Balb/c mouse models of asthma established using OVA received intraperitoneal injections of chloroquine, dexamethasone, or both prior to OVA challenge. Within 24 h after the final challenge, airway hyper-responsiveness (AHR) of the mice was assessed, and the total cell count and the counts of different cell populations in the bronchoalveolar lavage fluid (BALF) were determined under light microscopy. The severity of lung inflammation was evaluated using HE staining, and the concentrations of IL-6 and PGF2α in the BALF were detected by enzyme-linked immunosorbent assay (ELISA). Results Chloroquine pretreatment significantly decreased AHR (P<0.001) in the asthmatic mice and reduced the total cell count (P<0.01), eosinophils (P<0.001), neutrophils (P<0.01), and PGF2α levels in the BALF. Chloroquine combined with low-dose dexamethasone significantly lessened inflammations around the bronchioles (P<0.05) and blood vessels (P<0.01) in the lung tissue, and obviously lowered IL-6 (P<0.05) and PGF2α (P<0.001) in the BALF in the asthmatic mice. Conclusion Chloroquine can inhibit AHR in asthmatic mice and produce better anti-inflammatory effect when combined with dexamethasone for treatment of neutrophilic asthma.
