Expression of chemokine receptor CXCR7 in gastric cancer tissues and cell lines
10.3969/j.issn.1673-4254.2014.12.15
- VernacularTitle:趋化因子受体7在胃癌组织及细胞中的表达及意义
- Author:
Ameng SHI
1
;
Lei DONG
;
Haitao SHI
;
Miao JIA
;
Xiaoyan GUO
;
Jiong JIANG
;
Bin QIN
Author Information
1. 西安交通大学医学院第二附属医院
- Keywords:
chemokine receptor;
CXCR7;
gastric cancer
- From:
Journal of Southern Medical University
2014;(12):1780-1784
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression status of CXCR7 in gastric cancer tissues and cell lines. Methods The expression status of CXCR7 was detected in 35 primary gastric cancer tissues and matched adjacent tissues by immunohistochemistry and RT-PCR. The correlation of CXCR7 expression with the clinicopathological parameters and risk factors of gastric cancer was analyzed. The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, BGC-823, SGC-7901 and MKN-28) was also detected by immunofluorescence assay. Results The expression of CXCR7 was significantly higher in gastric cancer tissues than in adjacent tissues (P<0.01). CXCR7 expression was not correlated with age, gender, smoking history, Helicobacter pylori infection, tumor location or the pathological type, but showed a higher expression level in patients with a alcohol-drinking history than in those without (P<0.05). CXCR7 was expressed with variable intensities in the 5 gastric cancer cell lines without correlation with the degrees of cell differentiation;its expression was the highest in SGC-7901 cells, a moderately differentiated human gastric adenocarcinoma cell line. Conclusions CXCR7 is highly expressed in gastric cancer tissues with variable intensities in 5 gastric cancer cell lines, suggesting its important role in gastric cancer progression.
