Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells

VernacularTitle:基于3'RACE的胃癌细胞肿瘤相关基因的APA位点分析
Author: Dengpan LAI ¹ ; Jian CHEN ; Yani KANG
Author Information

1. 上海交通大学系统生物医学教育部重点实验室
Keywords: gastric cancer; 3'RACE; alternative polyadenylation
From: Journal of Southern Medical University 2014;(12):1738-1742
CountryChina
Language:Chinese
Abstract: Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells. Methods We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90αand SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.