Establishment of a method for detecting peripheral blood circulating brain microvascular endothelial cells, a novel biomarker for blood-brain barrier injury

VernacularTitle:外周血中血脑屏障损伤新型标记物脑微血管内皮细胞检测方法的建立
Author: Yan LI ¹ ; Lei DU ; Lin YUAN ; Dexi CHEN ; Jiawen QIU ; Xiaolong HE ; Hong CAO ; Shenghe HUANG
Author Information

1. 南方医科大学公共卫生与热带医学学院微生物学系
Keywords: peripheral blood; biomarker; circulating brain microvascular endothelial cells; blood-brain barrier injury
From: Journal of Southern Medical University 2014;(12):1733-1737
CountryChina
Language:Chinese
Abstract: Objective To establish a method for detecting circulating brain microvascular endothelial cells (cBMECs), a novel biomarker of blood-brain barrier (BBB) injury. Methods Blood samples were collected from 33 patients with AIDS encephalitis and 13 healthy subjects for detection of cBMECs, cECs and EPCs using magnetic affinity isolation and immune identification technology. Results The numbers of cBMECs, cECs and EPCs were significantly higher in the AIDS patients than in the control subjects (t=4.298, P<0.01; t=4.886, P<0.01; t=4.889, P<0.01). An significant association was also noted between HIV load and cBMEC number (r=0.928, P<0.01). Conclusion We have successfully established a method for detecting peripheral blood cBMECs, which can be of important value in non-invasive assessment of BBB injury.