Effects of sevoflurane preconditioning on cardiomyocyte apoptosis and myocardial inflammation in rats with sepsis
10.3969/j.issn.1673-4254.2014.11.26
- VernacularTitle:不同吸入浓度七氟醚预处理对脓毒症大鼠心肌细胞凋亡及炎性反应的影响
- Author:
Junsong LAI
1
;
Liangcheng ZHANG
;
Huishu WANG
;
Pengtao LIN
;
Wenhua CHEN
Author Information
1. 福建医科大学附属协和医院麻醉科
- Keywords:
sevoflurane;
sepsis;
cardioprotection;
apoptosis;
inflammation
- From:
Journal of Southern Medical University
2014;(11):1680-1683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of preconditioning with different concentrations of sevoflurane on cariomyocyte apoptosis and myocardial inflammation in rats with sepsis and explore the possible mechanism of sevoflurane for myocardial protection. Methods Forty adult male Sprague-Dawley rats were randomly divided into 4 groups (n=10), namely the control group, LPS group, low-concentration sevoflurane group and high-concentration sevoflurane group. Following sevoflurane pretreatment for 30 min and a washout period for 10 min, all the rats received intraperitoneal injection of LPS or normal saline (NS) and were sacrificed 12 h later to observe the myocardial histopathology. Apoptosis of the ardiomyocytes was detected with TUNEL assay, and enzyme-linked immunosorbent assay was used to detect serum cTnI level and myocardial TNF-αlevel. Results Compared with the control group, the rats in the other 3 groups showed significantly increased serum cTnI level, myocardial TNF-α content, and apoptotic index of the cardiomyocytes (P<0.05). Compared with those in LPS group, serum cTnI level, myocardial TNF-α content, and apoptotic index of the cardiomyocytes were significantly decreased in the two sevoflurane preconditioning groups (P<0.05), and the effect was more obvious with a high dose of sevoflurane (P<0.05). Conclusion Sevoflurane preconditioning can concentration-dependently reduce LPS-induced myocardial injury in rats possibly by decreasing cardiomyocyte apoptosis and alleviating myocardial inflammations.
