Compound Huang Gan delays chronic renal failure after 5/6 nephrectomy in rats
10.3969/j.issn.1673-4254.2014.11.22
- VernacularTitle:复方黄甘延缓5/6肾切除大鼠慢性肾功能衰竭
- Author:
Xiaoyan XIAO
1
;
Liqian MO
;
Shaolian SONG
;
Min QIN
;
Xixiao YANG
Author Information
1. 南方医科大学南方医院药学部
- Keywords:
chronic renal failure;
5/6 nephrectomy;
compound Huang Gan;
glomerulosclerosis;
interstitial fibrosis
- From:
Journal of Southern Medical University
2014;(11):1661-1667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of compound Huang Gan in delaying chronic renal failure in rats after 5/6 nephrectomy and explore the possible mechanisms. Methods High-performance liquid chromatography was used to was used identify the components of compound Huang Gan extract. Rat models of 5/6 nephrectomy received a 12-week treatment with intragastric administration of Niaoduqing, Cozaar, or compound Huang Gan at low, moderate or high doses (n=10). After the treatments, the rats were sacrificed for detecting Scr, BUN, Ucr and 24h UPr , pathological examination of the renal tissues, and determination of FN, MCP-1, and ICAM-1 expression levels in the renal tissues using RT-PCR and immunohistochemistry. Results The major chemical components of compound Huang Gan extract included glycyrrhizin (0.61%), paeonol (1.2%), aloe emodin (0.72%), rhein (0.85%), emodin (0.87%), chrysophanol (0.79%) and physcion (0.8%). Treatment with compound Huang Gan at low, moderate and high doses significantly reduced Scr, BUN, Ucr , Ccr and 24 h UPr levels (P<0.05), improved interstitial fibrosis and glomerulosclerosis, and reduced FN and ICAM-1 expressions (P<0.05) in rats following nephrectomy. Conclusion Compound Huang Gan can improve the renal function and lessen glomerulosclerosis and renal interstitial fibrosis to delay the progression of chronic renal failure in rat models of 5/6 nephrectomy.
