Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes
10.3969/j.issn.1673-4254.2014.11.17
- VernacularTitle:苯乙烯类菁染料pH荧光探针在细胞荧光成像中的应用及细胞毒性检测
- Author:
Lixia HUANG
1
;
Xiang LIU
;
Danting CUI
;
Yikai XU
;
Wenwei YOU
;
Yichen YAN
;
Xinwei LU
;
Ruiyuan LIU
Author Information
1. 南方医科大学 南方医院影像中心
- Keywords:
styrylcyanine;
pH fluorescent probe;
cell imaging;
cytotoxicity
- From:
Journal of Southern Medical University
2014;(11):1642-1645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging. Methods The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell. Results Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability. Conclusion The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
