Ethanol reduces neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos
10.3969/j.issn.1673-4254.2014.11.01
- VernacularTitle:酒精能够减少斑马鱼胚胎的神经前体细胞并抑制其神经元和神经胶质细胞的分化
- Author:
Guo YIN
1
;
Fang YAO
;
Xiaohui CHEN
;
Nan WANG
;
Huili WANG
;
Hongen CHANG
;
Zhaohu YUAN
;
Bingyi WU
Author Information
1. 南方医科大学 南方医院临床实验研究中心
- Keywords:
zebrafish;
neural progenitor cells;
glial cells;
neurons;
differentiation
- From:
Journal of Southern Medical University
2014;(11):1555-1561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of exposure to different concentrations of ethanol on neural progenitor cells and the differentiation of neurons and glial cells in zebrafish embryos. Methods Zebrafish embryos were exposed to 1%, 2%, and 2.5%(V/V) ethanol at 5 hpf by adding ethanol to the egg water. In situ hybridization and real-time PCR were used to detect the changes in the mRNA expression profiles of the markers of different cells to examine the effects of alcohol on neural development. Results The number of neural precursor cells, neurons and mature glial cells was significantly reduced in the zebrafish embryos following ethanol exposure, and this reduction became more prominent as the ethanol concentration increased. The expression of the early glial marker slc1a3a was down-regulated in the spinal cord but increased in the brain after exposure to increased ethanol concentrations. The expression of the mature glial markers was significantly lowered in response to exposure to increasing ethanol concentrations. Conclusion Ethanol can reduce neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
