siRNA-mediated CDK6 knockdown suppresses nasopharyngeal carcinoma cell growth and cell cycle transition in vitro
10.3969/j.issn.1673-4254.2014.07.33
- VernacularTitle:siRNA抑制CDK6表达影响鼻咽癌细胞增殖和细胞周期进展
- Author:
Xiaopeng LUO
1
;
Qiong XIA
;
Jixin QIN
;
Yongzhi HUANG
;
Jin LIU
;
Ying WANG
;
Huaifei WANG
;
Jiajun CHEN
Author Information
1. 右江民族医学院附属医院耳鼻喉科
- Keywords:
nasopharyngeal carcinoma;
CDK6;
small interfering RNA;
cell proliferation;
cell cycle
- From:
Journal of Southern Medical University
2014;(7):1071-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of small interfering RNA (siRNA)-mediated suppression of CDK6 expression on the proliferation and cell cycles of nasopharyngeal carcinoma (NPC) cells in vitro. Methods QRT-PCR was used to examine the differential expression of CDK6 in 30 NPC tissues and 18 normal nasopharyngeal tissues. A siRNA targeting CDK6 was transfected in NPC CNE2 cells, and MTT assay and flow cytometry were used to analyze the changes in cell proliferation and cell cycle distribution. Western blotting was used to examine the expressions of the cell cycle-related factors. Results Compared with normal nasopharyngeal tissues, NPC tissues showed an increased expression of CDK6 mRNA. Knocking down CDK6 expression obviously inhibited tumor cell growth and cell cycle transition from G1 to S phase and caused reduced expressions of CDK4, CCND1, and E2F1 and enhanced expression of the tumor suppressor p21. Conclusion NPC tissues overexpress CDK6. Knocking down CDK6 expression inhibits the growth and cell cycle transition of NPC cells in vitro by inhibiting the expressions of CDK4, CCND1, and E2F1 and upregulating tumor suppressor p21 expression.
