CD147 expression level and rs8259 T/A polymorphism of CD147 in patients with acute coronary syndrome
10.3760/cma.j.issn.0253-3758.2014.07.009
- VernacularTitle:急性冠状动脉综合征患者 CD147表达水平及其 rs8259 T／A 基因多态性分析
- Author:
Yu MAO
1
;
Jinchuan YAN
;
Cuiping WANG
;
Zhongqun WANG
;
Peijing LIU
;
Wei YUAN
Author Information
1. 江苏大学附属医院心内科
- Keywords:
Coronary disease;
Polymorphism,single nucleotide;
Antigens,CD147
- From:
Chinese Journal of Cardiology
2014;(7):566-570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between CD 147 expression and its untranslated regions 3′UTR rs8259 T/A polymorphism and acute coronary syndrome ( ACS).Methods The genotypes of CD147 were detected by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) methods in 182 ACS patients and 328 healthy controls.The plasma level of CD147 was determined by enzyme-linked immunosorbent assay (ELISA).CD147 mRNA and protein expression was detected by real-time fluorescent quantitative PCR ( RT-qPCR ) and Western blot.Results The plasma CD147 level obtained from radial artery in ACS patients ((3.63 ±0.70) pg/L) was significantly higher than in control ((2.45 ±0.27) pg/L, P<0.05), and highest in plasma obtained from the coronary artery ((4.28 ± 1.03)pg/L, P<0.05) in ACS patients.Furthermore, the plasma CD147 level was higher in the ACS patients with rs8259 AA genotype than in the ACS patients with rs 8259 TT genotype ((4.08 ±0.41) pg/L vs.(3.05 ±0.79) pg/L in radial artery and (5.29 ±0.62) pg/L vs.(3.13 ±0.52) pg/L in coronary artery, both P <0.05 ).There are an enhanced expression of CD 147 mRNA ( 2.45 times higher than control) and protein (3.66 ±1.56 vs.1.81 ±1.29) in PBMCs from ACS patients than that from controls (both P<0.05).The PBMCs CD147 mRNA and protein expression level were significantly higher in ACS patients with rs8259 AA genotype (mRNA:2.45 ±0.35, protein:1.63 ±0.16) compared to ACS patients with rs8259 TT genotype ( mRNA:1.69 ±0.15, protein:0.88 ±0.16, both P<0.05).Multiple logistic analysis showed that CD147 T allele ( AT +TT) was a protective factor to ACS ( OR =0.667, 95%CI 0.507-0.879, P<0.05).Conclusions The over-expression of CD147 is involved in the pathogenesis of ACS.The CD147 3′UTR rs8259 T allele may be a protective factor for ACS , its polymorphism can affect the CD147 protein expression in ACS patients.
