Association of human chorionic gonadotropin level in embryo culture media with early embryo development
10.3969/j.issn.1673-4254.2014.07.25
- VernacularTitle:人胚胎源性人绒毛促性腺激素在胚胎培养液中的水平与早期胚胎发育的关系
- Author:
Haiying WANG
1
;
Renli ZHANG
;
Dong HAN
;
Caixia LIU
;
Jiajie CAI
;
Yanling BI
;
Anmin WEN
;
Song QUAN
Author Information
1. 广东省人民医院/广东省医学科学院妇产科生殖医学科
- Keywords:
embryonic;
human chorionic gonadotropin;
spent culture media;
embryo development;
in vitro fertilization
- From:
Journal of Southern Medical University
2014;(7):1039-1041,1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development. Methods Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed. Results In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02). Conclusion ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.
