The diagnostic value of urine-based survivin mRNA test using reverse transcription-polymerase chain reaction for bladder cancer: a systematic review
- VernacularTitle:RT-PCR技术检测尿液中survivin mRNA对膀胱癌诊断价值的系统评价
- Author:
Xia YAN
1
;
Liu YA-LI
;
Yang KE-HU
;
Chen WEI
Author Information
1. 兰州大学
- Keywords:
Bladder neoplasm;
survivin mRNA;
reverse transcription-polymerase chain reaction;
diagnostic test;
systematic review
- From:Chinese Journal of Cancer
2010;29(4):487-494
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective: Survivin has gradually become an important target in diagnosis,prognosis prediction and treatment of tumor.There are many studies on urine-based survivin mRNA test using reverse transcription-polymerase chain reaction(RT-PCR)as a noninvasive examination for bladder cancer.However,its clinical value remains controversial.This study was to evaluate the diagnostic value of urine survivin mRNA detection with RT-PCR for bladder cancer by a systematic review of related studies.Methods: With the search terms such as bladder neoplasm,survivin,RT-PCR,sensitivity,specificity and diagnosis,we systematically searched through PubMed,EMBASE,SCI,Cochrane Library.Chinese Biomedical Literature Database(CBM),Chinese Scientific Journal Full-Text Database(CSJD),China Journal Full-Text Database(CJFD),Chinese Medical Association(CMA)digital periodicals and Google Scholar totally from January 1997 to April 2009 for diagnostic trials with RT-PCR detection of urine survivin mRNA for bladder cancer.The Quality Assessment of Diagnostic Accuracy Studies(QUADAS)items were used to evaluate the quality of the included studies.Meta-disc software was used to calculate outcome indicators.Results: Twenty-six studies,totally 2416 patients,were eligible.Meta-analysis showed that compared with pathologic examination,the summary values of sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and summary receiver operating characteristic curve(SROC)of urine-based survivin mRNA test using RT-PCR for bladder cancer were88%,94%,14.56,0.13 and 0.9736,respectively.Nested RT-PCR got the highest sensitivity,specificity and SROC and the values were 91%,95% and0.9805,respectively.The sensitivity and specificity of general RT-PCR were the second highest,which were 87% and 94%,respectively.The sensitivity of quantitative RT-PCR was 80% and the specificity was 93%.Conclusions:Comparing with pathologic examination,the sensitivity and specificity of urine-based survivin mRNA test using RT-PCR are relatively high.It can be used as an important adjunct method for cystoscope in early screening and postoperative monitoring of bladder cancer.
