Relationship and clinical significance of TGH-β1 expreession with Treg cell infiltration in hepatocellular carcinoma
- VernacularTitle:肝癌组织中TGF-β1表达与Treg浸润的关系及其临床意义
- Author:
Lin GUO-HE
1
,
2
;
Wang JUN
;
Li SHU-HONG
;
Wang JIN
;
Xu LI
;
Li SHENG-PING
Author Information
1. 华南肿瘤学国家重点实验室,广东,广州,510060
2. 中山大学肿瘤防治中心肝胆科,广东,广州,510060
- Keywords:
Liver neoplasm;
regulatory T cells(Treg);
transforming growth factor-β1(TGF-β1)
- From:Chinese Journal of Cancer
2010;29(4):442-447
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective: There are few studies about origins of regulatory T(Treg)cells increased in primary hepatocellular carcinoma(HCC)tissue.Studies showed that Treg cells could be induced by transforming growth factor-β1(TGF-β1),but the relation of TGF-β1expression and Treg cell infiltration is unclear in HCC tissue.This study was to investigate the expression of TGF-β1 and correlation between the expression of TGF-β1 and the amount of Treg cells in HCC,and to evaluate their clinical values in predicting the prognosis of HCC.Methods: EnVision immunohistochemistry was used to detect the expression of TGF-β1 and Foxp3 in 102 specimens of HCC tissue and paired adjacent non-tumor liver tissue.Results:of the 102 specimens of HCC,41 showed low TGF-β1expression and 61(59.8%)showed high expression; of the 102 specimens of adjacent non-tumor tissue,22 showed low TGF-β1 expression and 80(78.4%)showed high expression.The high expression rate of TGF-β1 was significantly lower in HCC than in adjacent non-tumor tissues(P=0.001).Average Foxp3+ cell density in HCC was 2.98 cells/HP,but there was very few or no expression of Foxp3 in adjacent non-tumor liver tissue.Expression of TGF-β1 was positively correlated with expression of Foxp3 in HCC tissuezs(r=0.228,P=0.021).The expression of TGF-β1 was significantly higher in HCC tissues with high preoperative AFP concentration than in those with low preoperative AFP concentration(P=0.023).TGF-β1 and Foxp3 expression had no correlations with tumor diameter,tumor capsule,liver cirrhosis,and so on.The 5-year survival rate was not different between HCC tissues with high and low TGF-β1 expression(P=0.790).It was significantly lower in HCC tissues with high Treg cell infiltration than in those with low infiltration(25% vs.44%,P=0.007).Cox multivariate analysis showed that the number of Treg cells and tumor capsule were independent prognostic factors(P=0.021,P=0.001).Conclusions: Expression of TGF-β1 may relate to the infiltration of Treg cells in HCC tissues,but the relation need to be further investigated.The number of Treg cells in HCC tissues could be used as.a potential immunological prognostic indicator for HCC patients after resection.
