Preparation and antitumor effects of nanovaccines with MAGE-3 peptides in transplanted gastric cancer in mice
- VernacularTitle:MAGE-3多肽纳米疫苗对小鼠胃癌种植瘤抑瘤效应研究
- Author:
Yang JUN
1
;
Li ZHI-HUA
;
Zhou JIA-JIA
;
Chen RU-FU
;
Chang LIANG-ZHENG
;
Zhou QUAN-BO
;
Yang LI-QUN
Author Information
1. 中山大学附属第二医院
- Keywords:
Peptide vaccine;
nanoparticles;
self-assembly;
gastric carcinoma;
antitumor effect
- From:Chinese Journal of Cancer
2010;29(4):393-398
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective: As a prospective vaccine carrier,nanoparticles can protect antigens from degradation and enhance immune response.This study prepared nanovaccines with MAGE-3-derived CD4+CD8+T cell epitope peptides,and investigated its character and antitumor effects on transplanted gastric cancer in mice.Methods: We adopted the self-assembly method to prepare peptide/chitosan conjugated with deoxycholic acid(chitosan-deoxycholic acid)nanoparticles.We observed the appearance of the chitosan-deoxycholic acid nanoparticles through a transmission electron microscope(TEM)and analyzed the peptide content and its release pattern by fluorescence spectrophotometry.We observed tumor-suppression efficacy in vivo through animal experiments.Results: We successfully prepared nanoparticles with MAGE-3 peptide antigen,and its encapsulation efficiency and loading level were about 37% and 17.0%,respectively.These nanoparticles presented a delayed release pattern in phosphate buffered saline(PBS)at pH 7.4,and the full release time was about 48 h.In 2 mg/mL lysozyme,the nanoparticles showed a sudden release,and the full release time was about 24 h.ELISPOT and cytotoxic experiments showed that the MAGE-3 peptide loaded nanoparticles could stimulate immune response in vivo and could generate MAGE-3-targeted cytotoxic T lymphocytes(CTLs),and kill MAGE-3-specific tumor cells.Tumor suppression experiments showed that the regression ratio of the peptide-loaded nanoparticles group was 37.81%.Conclusion: MAGE-3 peptide/chitosan-deoxycholic acid vaccine-loaded nanoparticles can stimulate antitumor immune response in vivo and can regress the growth of mouse forestomach carcinoma cell line MFC.
