Inhibitory effect of trichostatin A on HepG2 cell proliferation and the mechanisms
10.3969/j.issn.1673-4254.2014.07.01
- VernacularTitle:曲古抑菌素对HepG2细胞增殖的影响及相关机制
- Author:
Qingqiang SHI
1
;
Guowei ZUO
;
Ziqiang FENG
;
Lcui ZHAO
;
Nian LUO
;
Zhimei YOU
;
Jing XIA
;
Danyang LI
;
Jing LI
;
Dilong CHEN
Author Information
1. 重庆医科大学组织学与胚胎学教研室
- Keywords:
trichostatin A;
HepG2 cells;
apoptosis;
beta-catenin;
histone acetylation
- From:
Journal of Southern Medical University
2014;(7):917-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of trichostatin A (TSA) on the proliferation of HepG2 cells and explore the underlying mechanism. Methods HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72 h were examined for cell growth inhibition using a cell counting kit, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under inverted microscope. The expressions of beta-catenin, HDAC1, HDAC3, H3K9, cyclinD1 and Bax proteins in the exposed cells were detected by Western blotting, and the expressions of HDAC1 and HDAC3 mRNAs by quantitative fluorescent PCR. Results Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (P<0.05) and resulted in increased cell percentage in G0/G1 and G2/M phases and decreased cell percentage in S phase. The apoptotic index in the control group was (6.22 ± 0.25)%, which increased to (7.17 ± 0.20)%and (18.14 ± 0.42)%after exposure to 250 and 500 nmol/L TSA, respectively. Exposure to 250 and 500 nmol/L TSA also caused cell morphology changes with numerous floating cells. The expressions of beta-catenin, H3K9 and Bax proteins were significantly increased and CyclinD1, HDAC1, and HDAC3 protein expressions decreased in TSA-treated cells, but the expressions of HDAC1 and HDAC3 mRNAs showed no significant changes. Conclusion TSA can inhibit the proliferation of HepG2 cells and induce cell cycle arrest and apoptosis by inhibiting HDAC activity, promoting histone acetylation, and activating Wnt/beta-catenin signaling pathway.
