Diagnostic values of salivary versus and plasma microRNA-21 for early esophageal cancer
10.3969/j.issn.1673-4254.2014.06.26
- VernacularTitle:唾液与血浆微小RNA-21对早期食管癌的诊断价值
- Author:
Minhua YE
1
;
Penghui YE
;
Weizhu ZHANG
;
Jiaqi RAO
;
Zijun XIE
Author Information
1. 广东省人民医院/广东省医学科学院协和高级医疗中心
- Keywords:
esophageal neoplasms;
saliva;
diagnosis;
microRNA;
biological markers
- From:
Journal of Southern Medical University
2014;(6):885-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of salivary and plasma miR-21 in patients with esophageal cancer (EC). Methods Total RNA was extracted from saliva and plasma samples from 50 stage I and 50 stage II patients with EC and 50 healthy controls for measurement of miR-21 levels using qPCR. The diagnostic values of salivary and plasma miR-21 levels were assessed for stage I, stage II, and stage I+II EC. Results Salivary and plasma miR-21 were significantly higher in the EC patients than in the control group. The diagnostic sensitivities of plasma miR-21 for stage I, stage II, and stage I+II EC were 96%, 64%and 97%, with specificities of 44%, 84%, and 56%, respectively;the sensitivities of salivary miR-21 were 90%, 88%, and 89%, respectively, with the same specificities of 64%. Regardless of EC staging, the expression of plasma miR-21 showed a significant positive correlation with that of salivary miR-21, and their diagnostic values were comparable. Conclusion Both salivary and plasmatic miR-21 can be sensitive biomarkers for EC, and salivary miR-21 detection has the potential to replace plasma detection for EC diagnosis.
