Expression and clinical significance of Syndecan-1 mRNA and HPA-1 mRNA in the colorectal cancer detected with real-time fluorescent quantitative reverse transcription polymerase chain reaction
- VernacularTitle:实时荧光定量逆转录聚合酶链反应检测结肠癌中Syndecan-1 m RNA和HPA-1 m RNA的表达及临床意义
- Author:
Wang HE
1
;
Si JUN-LI
;
Zhang XIU-ZHEN
;
Qi YU-QIN
;
Niu ZI-YU
;
Zhou CHANG-HONG
Author Information
1. 青岛大学医学院附属青岛市市立医院
- Keywords:
Syndecan-1;
HPA-1;
colorectal neoplasm.invasion and metastasis;
real-time fluorescent quantitative reverse transcription polymerase chain reaction;
prognosis
- From:Chinese Journal of Cancer
2010;29(3):310-315
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective:Invasion and metastasis are the most common causes of mortality for patients with colorectaI neoplasms,and blocking invasion and metastasis in a timely fashion has become a hot research focus.We investigated the expression of the messenger RNA (mRNA)of Syndecan-1 and HPA-1 in colorecta J cancer,and their correlation with invasion and metastasis.Methods:Real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression of Syndecan-1 and HPA-1 in specimens from 49 patients with colorectal cancer.49 paired adjacent colorectal neoplasms(2 cm from the carcinoma),and 49 surgical margins of paired normal colorectal mucosa tissue(5 cm from the carcinoma),to analyze their correlation with clinicopathologic characteristics of colorectal neoplasm.Results:The expression of HPA-1 mRNA was significantly higher in colorectal cancer (40.56±11.75)than that in the paired adjacent colorectal neoplasms(18.28±11.33)and normal colorectal mucosa tissue(10.80±1020)(all P＜0.001).The expression of HPA-1 mRNA was significantly higher in paired adjacent colorectal neoplasms than that in normal colorectal mucosa(P＜0.05).The expression of Syndecan-1 mRNA was significantly higher in normal colorectal mucosa(61.21±12.96)than in the paired adjacent mucosa (14.35±11.06)or colorectaI cancer(10.12±8.58)(all P＜0.001).The expression of Syndecan-1 mRNA was significantly higher in the paired adjacent mucosa than that in colorectal cancer(P＜0.05).The decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 were closely associated with the degree of differentiation,the depth of infiltration,lymph node metastasis,vessel metastasis,and TNM staging of colorectal cancer(all P＜0.05).Spearman rank correlation analysis demonstrated a significant correlation between Syndecan-1 and HPA-1(r=-0.405,P＜0.05).Conclusions:The expression of Syndecan-1 mRNA was significantly highest in normal colorectal mucosa and the expression of HPA-1 mRNA was significantly highest in colorectal cancer.At the same time,the decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 mRNA can promote the invasion and metastasis of colorectal cancer.The determination of Syndecan-1 and HPA-1 may be of value in the treatment as well as in the prognosis of patients with colorectal cancer.
