Promoter methylation of CHFR gene in gastric carcinoma tissues detected using two methods
- VernacularTitle:两种方法检测胃癌患者CHFR基因启动子区甲基化的状态
- Author:
Cheng ZHAO-DONG
1
;
Hu SHI-LIAN
;
Sun YU-BEI
;
Xu WEI-PING
;
Shen GAN
;
Kong XIANG-YONG
Author Information
1. 安徽医科大学附属省立医院
- Keywords:
CHFR gene;
methylation;
gastric neoplasm;
methylation-specific polymerase chain reaction (MSP);
combined bisulfite restriction analysis (COBRA)
- From:Chinese Journal of Cancer
2010;29(2):171-175
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective:Transcriptional silencing induced by CpG island methylation is believed to be one of the important mechanisms of carcinogenesis. Checkpoint with fork head-associated and ring finger (CHFR) governs the transition from prophase to prometaphase in response to mitotic stress.This study was to analyze the relationship between the methylation of CHFR gene and the clinicopalhologic features of gastric cancer, and the difference of results between methylation-specific polymerase chain reaction(MSP)and combined bisulfite restriction analysis (COBRA)in detecting aberrant methylation of CHFR gene in gastric cancer.Methods: Both MSP and COBRA methods were used to detect the promoter methylation of CHFR gene in gastric cancer specimens from 64 patients.The relationship between methylation status of CHFR gene and the clinicopathologic features of gastric cancer were analyzed using SPSS1 6.0.Results:The methylation rates of CHFR gene promoter were significantly higher in gastric cancer samples than in the corresponding paracancer normal gastric mucosa by MSP(51.6%MS.18.8%,P<0.001).However,there was no significant correlation between methylation status of CHFR gene and the clinicopathologic parameters of gastric cancer, including age,gender,tumor size,clinical stage,Borrman type,tumor invasion depth,differentiation,and lymph node metastasis(P>0.05).Aberrant methylation of the CHFR gene was detected in 27(42.2%) of the 64 specimens of gastric cancer using COBRA, which did not significantly differ from that using MSP(P>0.05).Conclusions:Aberrant methylation of the CHFR gene is a frequent event in the carcinogenesis of gastric cancer.Detecting the methylation of CHFR gene in gastric mucosa may conduce to the diagnosis of gastric cancer. No difference was found between MSP and COBRA in detecting promoter methylation of CHFR gene in gastric cancer.
