Effects of bone marrow- derived mast cells on expressions of type II collagen and glycosaminoglycan in co-cultured chondrocytes
10.3969/j.issn.1673-4254.2014.05.15
- VernacularTitle:骨髓源性肥大细胞对软骨细胞表达Ⅱ型胶原及糖胺多糖的影响
- Author:
Qingqing OUYANG
1
;
Jinjun ZHAO
;
Min YANG
Author Information
1. 南方医科大学南方医院风湿免疫科
- Keywords:
bone marrow-derived mast cells;
chondrocytes;
type II collagen;
compound 48/80;
glycosaminoglycan
- From:
Journal of Southern Medical University
2014;(5):669-673
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of the bone marrow-derived mast cells (BMMCs) on the expression of type II collagen and glycosaminoglycan (GAG) in chondrocytes co-cultured with BMMCs. Methods Primarily cultured mouse BMMCs at 4 weeks and the second passage of chondrocytes were plated in a Transwell co-cultured system at a ratio of 1∶10 in the presence or absence of sodium cromoglycate (DSCG) or compound 48/80 (C48/80). The chondrocytes were harvested and lysed for detecting type II collagen expression with ELISA and Western blotting and GAG expression using 1,9 dimethylmethylene blue (DBM). Results After a 24-hour culture, the chondrocytes co-cultured with BMMCs showed similar expression levels of type II collagen and GAG to the control group regardless of the presence of DSCG (P>0.05). Compared with chondrocytes cultured alone or with BMMCs, the co- cultured chondrocytes in the presence of C48/80 showed significantly lower expressions of type II collagen and GAG (P<0.01). Such results did not vary significantly as the culture time was extended to 48 h. Conclusions C48/80-activated BMMCs can reduce the expression of type II collagen and GAG in chondrocytes in the co-culture system.
