Correlation of hypermethylation of TSP1 gene with TGF-β1 level and T cell immunity in gastric cardia adenocarcinoma
10.3321/j.issn:1000-467X.2009.12.012
- VernacularTitle:贲门腺癌组织中TSP1高甲基化与TGF-β1及细胞免疫水平的关系
- Author:
Guo WEI
1
;
Dong ZHI-MING
;
Guo YAN-LI
;
Yang ZHI-BIN
;
Kuang GANG
;
Shan BAO-EN
Author Information
1. 河北医科大学第四医院
- Keywords:
gastric cardia adenocarcinoma;
DNA methylation;
TSP1 gene;
TGF-β1 gene;
T cell immunity
- From:Chinese Journal of Cancer
2009;28(12):1298-1303
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective:Thrombospondin-1(TSP1)is an inhibitor of angiogenesis and its promoter hypermethylation has been found resulting in gene silencing in some primary human carcinomas.This study was to investigate the promoter methylation of TSP1 and its correlation with TGF-β1 level and T cell lmmunity in gastric cardia adenocarcinoma(GCA).Methods:Methylation specific polymerase chain reaction(MSP)approach and immunohistochemistry method were used to examine the methylation status of the 5'CpG island and expression of TSP1 protein, respectively.The Ievel of TGF-β1 was measured by ELISA and T cell immunity of GCA by flow cytometry analysis.Results:TSP1 methylation frequency was significantly higher in tumor specimens than in corresponding normal tissues(35.4% vs.3.1%,P<0.001)and significanty higher in Stages Ⅲ and Ⅳ tumor tissues than in Stages Ⅰ and Ⅱ tumor tissues(P<0.05).TSP1 protein expression was significantly lower in the tumor tissues than in corresponding normal tissues (P<0.05)and statistically correlated with its methylation status(P0.05).The function of T cell immunity was significantly different between the GCA patients with hypermethylation of TSP1 and those without methylation of TSP1(P<0.05).Conclusion:Promoter hypermethylation of TSP1 may play an important role in the development of GCA and reflect the biological behaviours of GCA.
