Gene analysis of polymerase basic protein 2 variant strains of influenza virus H1N1pdm09 subtype in Guangdong province
10.3760/cma.j.cn112866-20240521-00084
- VernacularTitle:广东省H1N1pdm亚型流感病毒聚合酶碱性蛋白2变异株基因分析
- Author:
Lijun LIANG
1
;
Qianfang GUO
;
Yushi HUANG
;
Jianxiang YU
;
Lirong ZOU
;
Huan ZHANG
;
Haiyan WANG
;
Zhencui LI
;
Baisheng LI
Author Information
1. 广东省疾病预防控制中心病原微生物检验所，广州 511430
- Keywords:
H1N1pdm;
Polymerase basic protein 2;
Mutation
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(5):558-563
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the molecular characteristics of the mutant strain of polymerase basic protein 2 (PB2) gene of influenza A (H1N1pdm) in Guangdong province, and to explore its specific molecular sites, so as to provide scientific basis for the prevention and control of influenza virus.Methods:Throat swab samples were collected from 2 cases infected with PB2 gene variant strains for virus isolation, and 23 influenza virus strains were selected from Guangdong province for sequencing analysis. The reference sequences and vaccine strain sequences provided by GISAID were used to perform evolutionary analysis on hemagglutinin (HA) and PB2 genes. Virus strain antigen analysis and neuraminidase (NA) inhibition test were carried out. PB2 protein model was constructed and polymerase activity was analyzed.Results:H399N amino acid mutation occurred in the HA gene of PB2-D701N and PB2-A271S variant strains, both of which belonged to the branch of 6B.1A.5a.2a. They belonged to the same big branch and different small branches as the vaccine strain A/Victoria/4897/2022, and they are all vaccine-like strains. In the three-dimensional structure, the mutations of PB2-D701N and PB2-A271S change charge and hydrophobicity.Conclusions:PB2-D701 and A271 were highly conserved, and PB2 mutant strains were not the dominant strains. The PB2 mutant had high antigenicity with the vaccine. The PB2 mutant strain is sensitive to NA inhibitors. The three-dimensional model predicted that PB2-D701N mutation could enhance virulence and affect transmissibility of influenza virus, while PB2-A271S mutation could affect polymerase activity and polymerase complex synthesis of influenza virus.
