Reversal effect of Fe_3O_4-magnetic nanoparticles on multi-drug resistance of ovarian carcinoma cells and its correlation with apoptosis-associated genes
10.3321/j.issn:1000-467X.2009.11.007
- VernacularTitle:磁性纳米Fe_3O_4对卵巢癌细胞耐药性的逆转作用及其与凋亡相关基因的关系
- Author:
Jiang ZHI
1
;
Chen BAO-AN
;
Wu QIANG
;
Xia GUO-HUA
;
Zhang YU
;
Gao FENG
;
Hong TIE-YAN
;
Xu CUI-RONG
;
Cheng JIAN
;
Li GUO-HONG
;
Chen WEN-JI
;
Liu LI-JIE
;
Li XIAO-MAO
;
Wang XUE-MEI
Author Information
1. 东南大学临床医学院
- Keywords:
Fe_3O_4-magnetic nanoparticles;
ovarian neoplasm;
SKOV3/DDP cells;
multi-drug resistance;
reverse;
apoptosis-associated genes
- From:Chinese Journal of Cancer
2009;28(11):1158-1162
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective: Resistance to cisplatin (DDP)remains a major obstacle for the successful treatment of ovarian cancer.This study was to determine the reversal effect of Fe_3O_4-magnetic nanoparticles (MNPs)on DDP-resistance of ovarian carcinoma cell line SKOV3/DDP,and to explore its correlation with apoptosis-associated genes.Methods: SKOV3/DDP cells were divided into the DDP group,the Fe_3O_4-MNPs group, the combination (DDP plus Fe_3O_4-MNPs) group,and the control group. Cell proliferation was determined by MTT assay.Cell apoptosis was analyzed by flow cytometry (FCM).Intracellular DDP level was detected by inductively coupled plasma atomic emission spectrometry(ICP-AES).The expressions of apoptosis-associated genes, bcl-2, and survivin were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results:Fe_3O_4-MNPs reversed DDPresistance of SKOV3/DDP cells by 2.259 folds.The cell apotosis rate and the intracellular DDP level were significantly higher in the combination group than in the DDP group (P＜0.05). Moreover,the mRNA levels of bcl-2 and survivin were significantly lower in the combination group than in the DDP group (P＜0.05). Conclusions:Fe_3O_4-MNPs can reverse the DDP resistance of ovarian carcinoma SKOV3/DDP cells, and the effect may be ascribed to the down-regulation of bcl-2 and survivin expression.
